Lipidomics Core Facility

The Lipidomics Core Facility is a research facility located at the Department of Biochemistry and Molecular Biology, at the University of Debrecen, Faculty of Medicine. The laboratory’s mission is to develope and optimize SRM-based targeted lipid identification and quantification methods with high emphasis on lipid metabolites enabling research principally in the Faculties of Science and Medicine of the University of Debrecen. The Lipidomics Core Facility undertakes research, collaborations and analytical services for other Universities and biotech companies in the lipidomics field providing access to state-of the art lipid metabolite identification and quantification.

Instrumentation:
- Waters 2695 HPLC coupled to Micromass Quattro Ultima Platinium (Waters) triple quadrupole-type mass spectrometer and Waters 2998 Photodiode Array Detector operated by MassLynx V4.1 (Waters) software
- Skyline software for evaluation of SRM data

Services:
- Eicosanoid analysis
The following molecules can be examined parallely: arachidonic acid, prostaglandins such as PGE2, PGD2, PGF2α, 6-keto PGF1α, PGA2, PGB2, PGJ2 and thromboxane B2 (TXB2), 5-HETE and 15-HETE, oxidized lipids such as 9-HODE and 13-HODE
1, Analysis of pro- and anticoagultion balance: determination of PGI2:TXA2 ratio by analysing the amount of their stable metabolites, the 6-keto PGF1α originating from the instable PGI2 and the TXB2 originating from instable TXA2
2, Analysis of inflammation: quantification of different prostaglandins
3, Oxidized lipid quantification: quantification of 9-HODE and 13-HODE

- Endocannabinoid analysis
Quantitative analysis of anandamide, 2-arahcydonyl glycerol (2-AG), 1-arahcydonyl glycerol (1-AG), oleoyl-etanolamide (OEA) and palmytoyl-ethanolamide (PEA)

- Method development for lipid metabolite analysis