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Biomolecular Interactions Service Lab, Biacore

University of Debrecen, Faculty of Medicine, Department of Medical Chemistry,
Responsible for liaison: Bálint Bécsi junior research fellow
Contact person: Bálint Bécsi
Phone: +36 52 518600 /51165
E-mail address: bbalint@med.unideb.hu
Webpage:
Review
In the Biomolecular Interaction Laboratory we study and characterize the interaction of biomolecules using Biacore-3000 equipment based on the principles of surface plasmon resonance. To carry out such studies relatively low amount of the binding partners are needed. One of the binding partners is immobilized on the surface of a sensor chip by covalent coupling, then the solution containing the other partner is injected over the surface and the interaction is detected and quantified. The equipment is suitable for multi-chanell analyses at wide temperature (4-40°C) and flow rate (1-2 ml/min to 50-100 ml/min) range. It is also able to detect relatively weak (Ka~10^4) interactions. The equipment is supplied with a user friendly software which makes possible the evaluation of the binding data for different kinetic mechanisms.